Smithsonian Research Bibliography

Pukazhenthi, Budhan, Quse, Viviana, Hoyer, Mark, Gastelaars, Heleen Van Engeldorp and Brown, Janine L. 2013. A review of the reproductive biology and breeding management of tapirs (Tapirus sp.). Integrative Zoology, 8(1): 18-34. doi:10.1111/j.1749-4877.2012.12008.x

Jewgenow, K., Pukazhenthi, B. S. and Schoen, J. 2012. Analysis of Sertoli cell efficiency allows the differentiation between two fundamentally different forms of feline teratospermia. Theriogenology, 79(2): 261-266. doi:10.1016/j.theriogenology.2012.08.014

Kiso, Wendy K., Asano, Atsushi, Travis, Alexander J., Schmitt, Dennis L., Brown, Janine L. and Pukazhenthi, Budhan S. 2012. Pretreatment of Asian elephant (Elephas maximus) spermatozoa with cholesterol-loaded cyclodextrins and glycerol addition at 4°C improves cryosurvival. Reproduction, Fertility and Development, 24(8): 1134-1142. doi:10.1071/RD11266

Stewart, Rosemary A., Pelican, Katharine M., Crosier, Adrienne E., Pukazhenthi, Budhan S., Wildt, David E., Ottinger, Mary Ann and Howard, JoGayle. 2012. Oral Progestin Priming Increases Ovarian Sensitivity to Gonadotropin Stimulation and Improves Luteal Function in the Cat. Biology of reproduction, 87(6): 137 doi:10.1095/biolreprod.112.104190

Vansandt, L. M., Pukazhenthi, Budhan S. and Keefer, C. L. 2012. Molecular Markers of Spermatogonial Stem Cells in the Domestic Cat. Reproduction in Domestic Animals, 47(Supplement S6): 256-260. doi:10.1111/rda.12079

Vick, Mandi,M., Wildt, David E., Turner, Jessica B., Palme, Rupert, Wolfe, Barbara A. and Pukazhenthi, Budhan S. 2012. Glucocorticoid response to changes in enclosure size and human proximity in the Persian onager (Equus hemionus onager). Stress, 15(1): 52-61. doi:10.3109/10253890.2011.578267

Comizzoli, Pierre, Pukazhenthi, Budhan and Wildt, David E. 2011. The competence of germinal vesicle oocytes is unrelated to nuclear chromatin configuration and strictly depends on cytoplasmic quantity and quality in the cat model. Human Reproduction, 26(8): 2165-2177. doi:10.1093/humrep/der176

Kiso, Wendy K., Brown, Janine L., Siewerdt, Frank, Schmitt, Dennis L., Olson, Deborah, Crichton, Elizabeth G. and Pukazhenthi, Budhan. 2011. Liquid Semen Storage in Elephants (Elephas maximus and Loxodonta africana): Species Differences and Storage Optimization. Journal of andrology, 32(4): 420-431. doi:10.2164/jandrol.110.011460

Pukazhenthi, Budhan S., Hagedorn, Mary, Comizzoli, Pierre, Songsasen, Nucharin and Wildt, David E. 2011. Cryopreserving endangered species gametes, embryos and gonadal tissue: Challenges, successes and future directions. Cryobiology, 63(3): 308-309. doi:10.1016/j.cryobiol.2011.09.014

Pukazhenthi, Budhan, Togna, Gina Della, Padilla, Luis R., Smith, Diorene, Sanchez, Carlos R., Pelican, Katey and Sanjur, Oris I. 2011. Ejaculate Traits and Sperm Cryopreservation in the Endangered Baird's Tapir (Tapirus bairdii). Journal of Andrology, 32(3): 260-270. doi:0.2164/jandrol.110.011833

Pelican, K. M., Spindler, Rebecca E., Pukazhenthi, Budhan, Wildt, David E., Ottinger, M. A. and Howard, JoGayle. 2010. Progestin exposure before gonadotropin stimulation improves embryo development after in vitro fertilization in the domestic cat. Biology of Reproduction, 83(4): 558-567.

Siriaroonrat, B., Comizzoli, Pierre, Songsasen, Nucharin, Monfort, Steven L., Wildt, David E. and Pukazhenthi, Budhan. 2010. Oocyte quality and estradiol supplementation affect in vitro maturation success in the white-tailed deer (Odocoileus virginianus). Theriogenology, 73(1): 112-119.

Comizzoli, Pierre, Wildt, David E. and Pukazhenthi, Budhan. 2009. In vitro compaction of germinal vesicle chromatin is beneficial to survival of vitrified cat oocytes. Reproduction in Domestic Animals, 44: 269-274.

Godard, N. M., Pukazhenthi, Budhan, Wildt, David E. and Comizzoli, Pierre. 2009. Paracrine Factors from Cumulus-Enclosed Oocytes Ensure the Successful Maturation and Fertilization in Vitro of Denuded Oocytes in the Cat Model. Fertility and sterility, 91(5): 2051-2060.

Jewgenow, Katarina, Neubauer, Katrin, Blottner, Steffen, Schon, Jennifer, Wildt, David E. and Pukazhenthi, Budhan. 2009. Reduced Germ Cell Apoptosis During Spermatogenesis in the Teratospermic Domestic Cat. Journal of andrology, 30(4): 460-468. doi:10.2164/jandrol.108.006726

Schoen, J., Neumann, S., Wildt, David E., Pukazhenthi, Budhan and Jewgenow, K. 2009. Localization of estrogen receptors in the epididymis during sexual maturation of the domestic cat. Reproduction in Domestic Animals, 44: 294-301.

Songsasen, Nucharin, Fickes, A., Pukazhenthi, Budhan and Wildt, David E. 2009. Follicular morphology, oocyte diameter and localization of fibroblast growth factors in the domestic dog ovary. Reproduction in Domestic Animals (Supplement 2), 44: 65-70.

Wildt, David E., Comizzoli, Pierre, Pukazhenthi, Budhan and Songsasen, Nucharin. 2009. Lessons from biodiversity - the value of nontraditional species to advance reproductive science, conservation, and human health. Molecular reproduction and development, 77(5): 397-409.

Wildt, David E., Howard, JoGayle, Pelican, K., Brown, J. and Pukazhenthi, Budhan. 2009. Contributions of reproductive science to wild felid conservation. In: Breitenmoser, U., Iberian Lynx Ex-situ Conservation: An interdisciplinary approach.. Madrid, Spain: Fundación Biodiversidad, pp.

Baudi, D. L. K., Jewgenow, K., Pukazhenthi, Budhan, Spercoski, K. M., Santos, A. S., Reghelin, A. L. S., Candido, M. V., Javorouski, M. L., Muller, G. and Morais, R. N. 2008. Influence of cooling rate on the ability of frozen-thaw spermatozoa to bind to heterologous zona pellucida as assessed by competitive in vitro binding assays in the Ocelot (Leopardus pardalis) and Tigrina (Leopardus tigrinus). Theriogenology, 69: 204-211.

Comizzoli, Pierre, Wildt, David E. and Pukazhenthi, Budhan. 2008. Impact of anisosmotic conditions on structural and functional integrity of cumulus-oocyte complexes at the germinal vesicle stage in the domestic cat. Molecular reproduction and development, 75: 345-354.

Crosier, Adrienne E., Henghali, Josephine N., Howard, JoGayle, Pukazhenthi, Budhan, Terrell, Kimberly A., Marker, Laurie L. and Wildt, David E. 2008. Improved Quality of Cryopreserved Cheetah (Acinonyx jubatus) Spermatozoa after Centrifugation Through Accudenz. Journal of Andrology, 30(3): 298 doi:10.2164/jandrol.108.006239

Crosier, Adrienne E., Marker, Laurie, Howard, JoGayle, Pukazhenthi, Budhan, Henghali, Josephine N. and Wildt, David E. 2007. Ejaculate traits in the Namibian cheetah (Acinonyx jubatus): influence of age, season, and captivity. Reproduction, Fertility, and Development, 19: 370-382.

Kim, Y., Selvaraj, V., Pukazhenthi, Budhan and Travis, A. J. 2007. Effect of donor age on success of spermatogenesis in feline testis xenografts. Reproduction, Fertility, and Development, 19(7): 869-876.

Pukazhenthi, Budhan, Santymire, Rachel, Crosier, Adrienne E., Howard, JoGayle and Wildt, David E. 2007. Challenges in cryopreserving endangered mammal spermatozoa: morphology and the value of acrosomal integrity as markers of cryo-survival. Society for Reproduction and Fertility (Supplement), 65: 433-446.

Collins, C. W., Songsasen, Nucharin, Monfort, Steven L., Bush, R. Mitchell, Wolfe, B., James, S. B., Wildt, David E. and Pukazhenthi, Budhan. 2006. Seminal traits of the Przewalski's horse (Equus ferus przewalski) following electroejaculation. Animal Reproduction Science, 94: 46-49.

Comizzoli, Pierre, Wildt, David E. and Pukazhenthi, Budhan. 2006. Poor centrosomal function in cat testicular spermatozoa impairs embryo development in vitro after intracytoplasmic sperm injection. Biology of Reproduction, 75: 252-260.

Comizzoli, Pierre, Wildt, David E. and Pukazhenthi, Budhan. 2006. In vitro development of domestic cat embryos following intra-cytoplasmic sperm injection with testicular spermatozoa. Theriogenology, 66: 1659-1663.

Crosier, Adrienne E., Pukazhenthi, Budhan, Henghali, Josephine N., Howard, JoGayle, Dickman, Amy J., Marker, Laurie and Wildt, David E. 2006. Cryopreservation of spermatozoa from wild-born Namibian cheetahs (Acinonyx jubatus) and influence of glycerol on cryosurvival. Cryobiology, 52: 169-181.

Pelican, K. M., Wildt, David E., Pukazhenthi, Budhan and Howard, JoGayle. 2006. Ovarian control for assisted reproduction in the domestic cat and wild felids. Theriogenology, 66: 37-48.

Pukazhenthi, Budhan, Comizzoli, Pierre, Travis, Alexander J. and Wildt, David E. 2006. Applications of emerging technologies to the study and conservation of threatened and endangered species. Reproduction, Fertility, and Development, 18: 77-90.

Pukazhenthi, Budhan, Laroe, D., Crosier, Adrienne E., Bush, L. M., Spindler, Rebecca E., Pelican, K., Bush, R. Mitchell, Howard, JoGayle and Wildt, David E. 2006. Challenges in cryopreservation of clouded leopard (Neofelis nebulosa) spermatozoa. Theriogenology, 66: 1790-1796.

Pukazhenthi, Budhan, Neubauer, K., Jewgenow, K., Howard, JoGayle and Wildt, David E. 2006. The impact and potential etiology of teratospermia in the domestic cat and its wild relatives. Theriogenology, 66: 112-121.

Comizzoli, Pierre, Pukazhenthi, Budhan, Howard, JoGayle and Wildt, David E. 2005. Collaborative Science and its Impact on Management and Conservation of Felid Species. Communique, December: 23-25.

Pukazhenthi, Budhan S. (ed.) 2004. Workshop on Eld's Deer Conservation and Restoration Bangkok, Thailand; Washington, D.C.: Zoological Park Organization Thailand; Smithsonian's National Zoological Park. (Second annual Meeting of the Eld's Deer Interest Group on Eld's Deer Conservation and Restoration November 10-12, 2003; ) 55 pages.

Comizzoli, Pierre, Wildt, David E. and Pukazhenthi, Budhan. 2004. Effect of 1,2-Propanediol Versus 1,2-Ethanediol on Subsequent Oocyte Maturation, Spindle Integrity, Fertilization, and Embryo Development In Vitro in the Domestic Cat. Biology of Reproduction, 71: 598-604.

Comizzoli, Pierre, Wildt, David E. and Pukazhenthi, Budhan. 2004. Oocyte quality and in vitro culture in the domestic cat. Assisted Reproductive Technology and Science, 4: 11-13.

Neubauer, K., Jewgenow, K., Blottner, S., Wildt, David E. and Pukazhenthi, Budhan. 2004. Quantity rather than quality in teratospermic males: a histomorphometric and flow cytometric evaluation of spermatogenesis in the domestic cat (Felis catus). Biology of Reproduction, 71: 1517-1524.

Pukazhenthi, Budhan and Wildt, David E. 2004. Which reproductive technologies are most relevant to studying, managing, and conserving wildlife? Reproduction, Fertility, and Development, 16(2): 33-46.

Comizzoli, Pierre, Wildt, David E. and Pukazhenthi, Budhan. 2003. Overcoming poor in vitro nuclear maturation and developmental competence of domestic cat oocytes during the non-breeding season. Reproduction, 126(6): 809-816.

Axner, E., Pukazhenthi, Budhan, Wildt, David E., Linde-Forsberg, C. and Spindler, Rebecca E. 2002. Creatinine phosphokinase in domestic cat epididymal spermatozoa. Molecular reproduction and development, 62: 265-270.

Hagedorn, Mary M., Lance, S. L., Fonseca, Dina M., Kleinhans, F. W., Artimov, D., Fleischer, Robert C., Hoque, A. T., Hamilton, M. B. and Pukazhenthi, Budhan. 2002. Altering the membranes of fish embryos with aquaporin-3: an essential step toward successful cryopreservation. Biology of Reproduction, 67: 961-966.

Pukazhenthi, Budhan, Spindler, Rebecca E., Wildt, David E., Bush, L. M. and Howard, JoGayle. 2002. Osmotic properties of spermatozoa from felids producing different proportions of pleiomorphisms: Influence of adding and removing cryoprotectant. Cryobiology, 44: 288-298.

Harnal, V. K., Spindler, Rebecca E., Monfort, Steven L., Pukazhenthi, Budhan, Bird, D. M. and Wildt, David E. 2001. Sperm capacitation in vitro in the Eld's deer. Theriogenology, 56: 399-413.

Pukazhenthi, Budhan, Wildt, David E. and Howard, JoGayle. 2001. The phenomenon and significance of teratospermia in felids. In: Concannon, P. W., England, G. C. W., Farstad, W., Linde-Forsberg, C., Verstegen, J. P. and Doberksa, C., Advances in Reproduction in Dogs, Cats and Exotic Carnivores. Colchester: Journals of Reproduction and Fertility, Ltd, pp.423-433.

Pukazhenthi, Budhan, Howard, JoGayle and Wildt, David E. 2000. Current knowledge about the reproductive biology of the clouded leopard. In: Fletchall, N., Clouded Leopard Species Survival Plan (SSP) Husbandry Guidelines. Bethesda, MD: American Zoo and Aquarium Association, pp.22-23.

Pukazhenthi, Budhan, Noiles, E., Pelican, K., Donoghue, Ann M., Wildt, David E. and Howard, JoGayle. 2000. Osmotic effects on feline spermatozoa from normospermic versus teratospermic donors. Cryobiology, 40: 139-150.

Spindler, Rebecca E., Pukazhenthi, Budhan and Wildt, David E. 2000. Oocyte metabolism predicts the development of cat embryos to blastocyst in vitro. Molecular reproduction and development, 56: 163-171.

Monfort, Steven L., Wolfe, B. A., Blumer, E. S., Atkinson, M. W., Spindler, Rebecca E., Pukazhenthi, Budhan, Bush, R. Mitchell, Wildt, David E., Roth, Terri L. and Morrow, C. J. 1999. Artificial insemination in the scimitar-horned oryx as a conservation and management tool. .

Pelican, K. M., Pukazhenthi, Budhan, Wildt, David E. and Howard, JoGayle. 1999. Discontinuous colloidal gradient centrifugation reduces sperm flagellum abnormalities but disrupts acrosomes in cat ejaculates. Biology of Reproduction Suppl., 60: 136

Pukazhenthi, Budhan, Pelican, K., Wildt, David E. and Howard, JoGayle. 1999. Sensitivity of domestic cat (Felis catus) sperm from normospermic versus teratospermic donors to cold-induced acrosomal damage. Biology of Reproduction, 61: 135-141.

Pukazhenthi, Budhan, Howard, JoGayle, Long, J. and Wildt, David E. 1998. Development of assisted reproductive technologies for the conservation of the clouded leopard (Neofelis nebulosa). .

Pukazhenthi, Budhan, Long, J. A., Wildt, David E., Ottinger, M. A., Armstrong, D. L. and Howard, JoGayle. 1998. Regulation of sperm function by protein tyrosine phosphorylation in diverse wild species. Journal of andrology, 19: 675-685.

Pukazhenthi, Budhan, Wildt, David E., Ottinger, M. A. and Howard, JoGayle. 1998. Inhibition of the domestic cat spermatozoa acrosome reaction and zona pellucida penetration by tyrosine kinase inhibitors. Molecular reproduction and development, 49: 48-57.

Pukazhenthi, Budhan, Wildt, David E., Ottinger, M. A. and Howard, JoGayle. 1996. Compromised sperm protein phosphorylation after capacitation, swim-up and zona pellucida exposure to teratospermic domestic cats. Journal of andrology, 17: 409-419.

Wildt, David E., Pukazhenthi, Budhan, Brown, Janine L., Monfort, Steven L., Howard, JoGayle and Roth, Terri L. 1995. Spermatology for understanding, managing and conserving rare species. Reproduction, Fertility, and Development, 7: 811-824.